Isolation and Characterization of Human Induced Pluripotent Stem Cells-Derived Mesenchymal Progenitors

Purpose: Isolating human induced pluripotent stem cells (hiPS)-derived mesenchymal progenitors as a new source of mesenchymal cells which can differentiate into different lineages like adipose and bone. Materials and Methods: After 7 days of hiPS1 culture on matrigle coated dishes, spindle like cells around colonies were removed by cell scraper. These cells that had mesenchymal like morphology was characterized after 4-6 passages. Mesenchymal cell surface markers CD73, CD90, CD105, CD29, CD44 and hematopoietic cell surface markers CD34 and CD45 were analyzed by flow cytometry and cells were differentiated to osteogenic and adipogenic lineages by defined medium. Results: flow cytometric analysis demonstrated that hiPS1 derived mesenchymal progenitors were %98.71 ± 0.14 CD44+, %98.51 ± 1.02 CD29+, %87.74 ± 3.41 CD105+, %46.65 ± 5.76 CD73+, %98.53 ± 0.78 CD90+and they did not express CD34 and CD45. These cells could be differentiated to osteogenic and adipogenic lineages. Conclusion: hiPS1 can make mesenchymal progenitors and these cells can be a suitable substitute for mesenchymal stem cells.